Figure 2
From: Combinatorial Atoh1 and Gfi1 induction enhances hair cell regeneration in the adult cochlea
Efficient HC ablation after DT injection and adenovirus scala media inoculation. Images show cochleae of adult mice 10 days after adenoviral inoculation of scala media with or without HC ablation. (a,a′,b,b′) Ad.Atoh1 adenovirus inoculation of wild-type (WT) mouse without DT-induced HC ablation. (c,c′,d,d′) Ad.Atoh1 adenovirus inoculation of Pou4F3DTR mouse with simultaneous DT injection. (e,e′,f,f′) adenovirus Ad.Gfi1.Atoh1 inoculation of Pou4F3DTR mouse with simultaneous DT injection. Dotted lines in a, b indicate where OHCs would be found in normal mice. In animals receiving only the injection surgery, most outer HCs are damaged and only a few OHCs remain in the most apical region, but inner HCs are largely intact. In contrast, all HCs both outer HCs and inner HCs, are ablated by simultaneous DT injection in both the Ad.Atoh1 group (c,d) and the Ad.Gfi1.Atoh1 group (e,f). All groups robustly expressed the tdTomato reporter (a′–f′). Myosin VIIa, Myosin VIIa. tdTom, tdTomato. Representative figures from 5 biological replicates for each group. Scale bar = 100 μm.
