Table 2 Primary antibodies.

From: Analysis of enteric nervous system and intestinal epithelial barrier to predict complications in Hirschsprung’s disease

Antibody

Host

Source or reference

Dilution

Method

HuC/D

Mouse

INVITROGEN, USA (A-21271)

1:200

Immunofluorescence

nNOS

Rabbit

ALEXIS BIOCHEMICALS, USA (ALX-210-501)

1:2000

Immunofluorescence

Calretinin

Rabbit

INVITROGEN, USA (18-0211)

1:500

Immunofluorescence

ZO-1

Rabbit

THERMOFISHER SCIENTIFIC, USA (40-2200)

1:200

Western Blot

Occludin

Rabbit

ABCAM, USA (ab167161)

1:50,000

Western Blot

Claudin-1

Rabbit

ABCAM, USA (ab180158)

1:200

Western Blot

JAMA

Rabbit

BETHYL LABORATORIES, USA (A 302891)

1:1000

Western Blot

Cingulin

Rabbit

SANTA CRUZ BIOTECHNOLOGY, USA (sc-66831)

1:500

Western Blot

panGFAP

Rabbit

DAKO, France (Z0334)

1:2000

Western Blot

GFAP N-term

Mouse

SCBT, USA (F-2)

1:500

Western Blot

β-Actine

Mouse

SIGMA

1:10,000

Western Blot

  1. HuC/D human neuronal protein, nNOS neuronal nitric oxide synthase, GFAP glial fibrillary acidic protein.