Figure 5 | Scientific Reports

Figure 5

From: Saturated fatty acids induce insulin resistance in podocytes through inhibition of IRS1 via activation of both IKKβ and mTORC1

Figure 5

Palmitate-induced insulin resistance is blocked by ceramide synthesis inhibitor. Expression of phospho-IRS1 (ser307), IRS1, phospho-Akt, Akt, phospho-IκBα (ser32/36), IκBα, phospho-S6 (ser240/244), S6, and actin were detected by (a) immunoblot and (b, c, d) densitometry quantitation was measured from mouse podocytes treated or not with myriocin 30 min prior the exposure of palmitate for 24 h, and then stimulated with insulin for 5 min. Results are shown as mean ± SD of 4 independent experience.

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