Figure 7
Wound area and depth are decreasing over time paralleled by progressing cytokine release. From day 1 to day 6 cytokine release increases over time (a–c), whereas wound area (d) and depth (e) tend to decrease. For IL-1β, debridement further increases cytokine release (a) whereas for IL-6 and IL-8 debridement has no influence (b,c). For IL-6 and IL-8, there is a tendency towards reduced cytokine release following neutrophil supplementation although this difference is not significant (b,c). If skin tissues are infected, debrided or both, neutrophil supplementation partially reduces wound area (d) and wound depth (e). For number of skin donors and technical replicates see Table S1. Colors represent various donors (a–c). Symbols are as follows: filled triangle, filled circle = non-debrided vs. open triangle, open circle = debrided, filled circle, open circle = non-infected vs. filled triangle, open triangle = infected, black = without neutrophil supplementation vs. grey with neutrophil supplementation (d,e). One-way ANOVA with Tukey’s post-hoc test testing day 1 vs. day 6 (asterisks without brackets) and without vs. with neutrophils (asterisks with brackets). Levels of significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
