Figure 3

Most axon defects occur at the juncture with the dorsal muscle. (A, B) juIs76 strain animals bearing the DD/VD neuron GFP marker were stained with Alexa 488-conjugated phalloidin antibody. Thus, both muscle and axons are stained in green. Axon defects on the dorsal side that occur between the muscles (A) or at the juncture with the muscle (B). Yellow arrow indicates defective axon. White arrow indicates normal axon. Blue arrowhead indicates dorsal muscle cell. Dorsal and ventral sides indicated. Scale bars indicate 50 μm. (C) Most axon defects occur at the juncture with the muscle (black) rather than between the muscles (grey striped).