Figure 4

Global factor analysis for mammary epithelial colony phenotype. A GLM model is developed for each phenotype, which provides a characteristic microenvironmental signature for (a) polarized, (b) non-polarized, (c) spread and (d) inverted colonies. LF: laminin + fibronectin, LC: laminin + collagen IV, CF collagen IV + fibronectin, LCF laminin + collagen IV + fibronectin. The numbers on the x-axis indicate the average proportion of each phenotype within each well, for the given factor within the category. Statistical analysis identifies the significance of the change with respect to the condition in light gray for every category. Error bars represent s.e.m. All pairwise differences were computed using the Tukey–Kramer method. ***P < 0.001, **P < 0.01, *P < 0.05.