Figure 1
From: Age related extracellular matrix and interstitial cell phenotype in pulmonary valves
Isolation of porcine PV leaflets and experimental design. (A) PV leaflets were isolated from female pigs with ages of 4–6 months and 2 years (denoted as young and adult, respectively). (B) isolated PV leaflets from young and adult pigs; (C) thickness of young and adult PV leaflets (measured around free edge of valve cusp, 5 PV leaflets from three pigs for each age group were used, **p < 0.01); (D) schematic illustration of experimental design in this study. The isolated PV leaflets were subjected to mechanical properties testing, histological staining, ECM component measurement, proteomic analysis, and PVIC isolation for RNA seq.
