Figure 2
From: Age related extracellular matrix and interstitial cell phenotype in pulmonary valves

Histological staining and quantitative analysis of ECM components of young and adult PV leaflets. (A) H&E and Movat's pentachrome staining (F fibrosa, S spongiosa, V ventricularis; arrows indicate elastin fibers); Quantitative analysis of collagen (B) and GAGs (C) by using hydroxyproline and DMMB assays, respectively (five PV leaflets from 3 different pigs, two pieces from each PV leaflets, **p < 0.01).