Figure 3 | Scientific Reports

Figure 3

From: The novel ECF56 SigG1-RsfG system modulates morphological differentiation and metal-ion homeostasis in Streptomyces tsukubaensis

Figure 3

SigG1 binding to target promoters in vivo and in vitro (a) Genome-wide distribution of SigG1 binding sites identified by ChIP-seq analyses using the anti-SigG1 polyclonal antibody. Peak calling was performed from two independent biological replicates. (b) Close-up of a 2 kb region around the target genes. S. tsukubaensis ΔrsfG: blue. Negative control (ΔsigG1): grey. Genes in each genomic context are depicted in green (c) MEME consensus for SigG1 DNA-binding motif obtained by aligning the sigG1-dependent ChIP-seq enriched sequences with the MUSCLE algorithm. The consensus logo was obtained using the WebLogo platform81. P1: STSU_23024p; P2: aldp; P3: STSU_22050; P4: wblCp; P5: STSU_32197p; P6: acnAp.

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