Figure 4
Phenotypes of the S. tsukubaensis wild-type, sigG1 and rsfG null mutants. (a) 106 spores were streak on ISP4 and grown for 14 days. To evaluate the phenotype of the complemented strains, 104 spores were plated on ISP4. The pIJ12333 vector was used to express sigG1 with its own promoter. The WT expressing the empty pIJ12333 is depicted as a control. (b) Differentiation into spores chains was examined by scanning electron microscopy (SEM), after 14 days of growth on ISP4. Scale bar: 2 µm.
