Figure 5 | Scientific Reports

Figure 5

From: The novel ECF56 SigG1-RsfG system modulates morphological differentiation and metal-ion homeostasis in Streptomyces tsukubaensis

Figure 5

Evaluation of metal-ion homeostasis in sigG1 mutants. (a) Chrome azurol S (CAS) assay to determine siderophore secretion in the supernatants of strains grown in iron limiting and iron-sufficient conditions (FeSO4 32 μM) conditions. (b) Intracellular iron content in S. tsukubaensis wild-type, ΔsigG1, ΔrsfG-sigG1 and respective complemented strains measured by flame atomic spectrometry (F-AAS). Values are representative of at least three independent experiments. (c) Phenotypes of the wild-type, sigG1 and rsfG-related strains grown on solid ISP4 supplemented with 100 µM CuSO4 medium for 6 days. Serial dilutions used were 105, 104, 103, 102, 101 spores. Statistically significant differences were determined by one-way ANOVA followed by a Dunnet’s test (GraphPad Prism).

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