Figure 3
From: A comparison of AAV-vector production methods for gene therapy and preclinical assessment
In vitro infectivity assay. Results from the in vitro transduction of HeLa cells for decreasing gc AAV-GFP vector produced from the Iod-GFP (A–j) and Chl-GFP (K–t) production methods. Representative low magnification images from the respective groups (Iod-GFP: A–j, Chl-GFP: K–t) clearly display the decrease in fluorescence intensity. High magnification confocal microscopy images from the centre of the wells (a–j and k–t) indicate that there are a few cells expressing GFP even at relatively low gc-titers. (U) Quantifications for actual cell numbers co-expressing DAPI and GFP. Each condition was repeated in triplicates. Note that a conservative threshold was chosen hence underestimating the true transduction rates.
