Table 1 eDNA site information on Christmas Island.

From: Under the karst: detecting hidden subterranean assemblages using eDNA metabarcoding in the caves of Christmas Island, Australia

Site no.

Site name

Type

Brief description

Salinity groups

1

19th Hole

Cave

Anchialine cave with tidal influence. Water table accessed on land via a small cave mouth and down a slope

Mesohaline

2

The Grotto

Cave

Marine coastal cave; strong sea currents and tidal influence. Accessed on land via Golf Course Road and open to the public. Possible connection to Whip Cave22

Oligohaline

3

Whip Cave

Cave

Anchialine cave system with tidal influence. Possible connection to The Grotto22

Oligohaline

4

Daniel Roux (Lower) Cave

Cave

Anchialine cave system with tidal influence. Water table accessed on land by the lower chamber entrance, down a series of steep rock-mounted steel ladders

Freshwater

5

Freshwater Cave

Cave

Anchialine stream passage cave connecting several chambers

Oligohaline

6

Hugh Dale Waterfall

Spring

Waterfall cascading from tufa spring

Freshwater

7

CI-079 (unnamed)

Spring

Small low cave with spring outflow

Freshwater

8

Sepulchral Soil Sink

Cave

Small entrance cave to deeper chambers with low pools

Mesohaline

9

Waterfall Spring

Spring

Water supply spring south of CI Resort

Freshwater

10

Freshwater Spring

Spring

Spring outflow north of CI Resort

Freshwater

11

Jedda Cave

Cave

Cement steps into doline, pump station for town water supply

Freshwater

12

Jane-up Cave

Cave

Muddy stream passage with high CO2; used for town water supply

Freshwater

13

Jones Spring

Spring

Spring with weir, water re-sinks nearby

Freshwater

14

Grants Well

Cave

Well shaft intersecting 20 m below to cave stream passage; high CO2

Freshwater

15

Thundercliff Cave

Cave

Sea cave entrance leading to freshwater outflow

Polyhaline

16

Ryan’s Ripper Rift

Cave

Rift cave ending in very deep crystalline edged anchialine water

Oligohaline

17

Hosnies Spring

Spring

Freshwater seepage on shore terrace that feeds a protected mangrove stand

Freshwater

18

Ross Hill Gardens Spring 1

Spring

Captured spring once used for town water supply

Freshwater

19

Ross Hill Gardens Spring 2

Spring

Captured spring once used for town water supply

Freshwater

20

Dolly Cave Spring

Spring

Low muddy cave with spring outflow

Freshwater

21

Lost Lake Cave 1

Cave

Extensive tidal system passages with various large breakdown chambers. Sampled from stream passage approximately 150 m from sea entrance

Oligohaline

22

Lost Lake Cave 2

Cave

Extensive tidal system passages with various large breakdown chambers. Sampled from stream passage far into the cave extent

Oligohaline

23

WiFi Cave

Cave

Deep vertical shaft that is believed to intersect the muddy stream passage between Grants Well and Jane-up Cave

Freshwater

  1. Type refers to whether samples at each respective site were taken from within a cave system or at a surface spring. Salinity classifications for each site were assigned based on salinity readings at the time of sample collection (see Table S1 for full environmental parameter data and CI cave index numbering).