Figure 2 | Scientific Reports

Figure 2

From: YY1 directly interacts with myocardin to repress the triad myocardin/SRF/CArG box-mediated smooth muscle gene transcription during smooth muscle phenotypic modulation

Figure 2

YY1 suppresses the transcription of VSMC genes encoding contractile proteins in PAC1 VSMCs. (A) PAC1 cells were transfected with YY1 expression plasmid (YY1) or the empty vector control (Ctr). The mRNA expression level of YY1, SMα-actin and SM22α was determined by qRT-PCR assays. (B) Knocking down YY1 by YY1 siRNA significantly increased the transcription of SMC-specific genes by qRT-PCR assays. Scr: the scramble siRNA as the control. (C) YY1 overexpression in PAC1 cells inhibited the promoter activity of SMα-actin-Luc, SM22α-Luc, SMMHC-Luc, but not ACLP-Luc by the luciferase assay. Values are mean ± SEM (n = 3) *P < 0.05, **P < 0.01 versus the control (Ctr).

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