Figure 3
From: Phage lysin that specifically eliminates Clostridium botulinum Group I cells
Effects of pH, NaCl, CaCl2 and MgSO4 on the lytic activity of CBO1751. Lytic activity analysis by measuring OD600 of Clostridium botulinum ATCC3502 frozen cells after addition of DB or 5 µM CBO1751 at pH from 4.5 to 11.5 (a) or in the presence of 12.5–400 mM NaCl (b), 0–200 mM CaCl2 (c) or 0–200 mM MgSO4 (d). All OD600 values were normalized to an initial OD600 of 1 and adjusted by subtraction of the corresponding DB control values. Results are indicated as means of three replicates ± standard deviations.
