Figure 4 | Scientific Reports

Figure 4

From: A protein of the metallo-hydrolase/oxidoreductase superfamily with both beta-lactamase and ribonuclease activity is linked with translation in giant viruses

Figure 4

Nuclease activity on various types of nucleic acids of expressed Tupanvirus protein (TupBlac) as assessed by dPAGE. Denaturant polyacrylamide gel electrophoresis (12% dPAGE) of nuclease activity on synthetic (+) and (−) single-stranded DNAs (130 nucleotide-long) (A), synthetic double-stranded DNA (B) (see Supplementary Table 3), or Escherichia coli RNA (B). Each part A and B of the figure correspond to different gels. No treatment (a); buffer (b); succinate dehydrogenase enzyme produced and purified by the same process and collected in the same fractions as Tupanvirus beta-lactamase TupBlac, used as negative control (c); Tupanvirus beta-lactamase TupBlac (d).

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