Figure 4

MALDI-imaging MS of sponges 1 day post-treatment. (a) The experimental isotopic patterns of aerophobin-2 (left panel) and aeroplysinin-1 (right panel) by MALDI-imaging MS. (b) The relative abundance of these compounds (Aero2 aerophobin-2, Aerop1 aeroplysinin-1) in the three different treatments (C control, G grazing, M mechanical damage) for each biological replicate (replicate; i.e., sponge individual). The corresponding microscopic images (Microsc) showing the location of the wounds are also shown, i.e. S surface (non-wounded); W1 the first evident wound; W2 the second evident wound, and so on. (c) Segmentation maps (SEG). Each cluster represents a chemical profile and is assigned a distinct colour that is comparable between treatments within each replicate. (d) Images of the co-localisation of aerophobin-2 and aeroplysinin-1 (AND), the distribution of at least one of these two compounds (OR, occurrence maps). The relative intensity from 0 to 100% is depicted in a colour scale with warmer colours representing relatively higher intensity and colder colours lower intensity of each compound. White-dotted line = broken or cut edges.