Figure 7

The chondrogenic assessment of avascular and vascular meniscal cells and chondrocytes. (a) GAG/DNA quantitation after 28 days of culture in pellets, a comparison of Avascular (Avas) and Vascular (Vas) meniscal cells and chondrocytes (Ch). Data shown are the means ± the standard deviation of triplicate runs and 6 donors for each cell population. (b) Histological analysis of a representative donor 6, chondrogenic pellet sections from Avas, Vas, Ch showing toluidine blue (TB), collagen type I (COL I) and collagen type II (COL II) staining. Scale bars represent 250 μm. (c) Collagen type I and (d) collagen type II semi-quantitative immunohistochemical (IHC) analysis, relative fold change to avascular meniscal pellet.