Figure 1

Soy-Tomato enriched diet reduces severity of chronic pancreatitis (CP). (A) C57BL/6 mice were injected intraperitoneally with 50 μg/kg caerulein (7 hourly injections, twice weekly) for 6 weeks. Mice across all groups were a fed a control diet from the start. After 4 weeks of injections, one group was fed a soy-tomato enriched dietary intervention (B) After 2 weeks of dietary intervention, severity of chronic pancreatitis was assessed from H&E and Trichrome stained pancreatic tissue at study endpoint. (C) Chronic pancreatitis index (score range 0–15) was expressed as a sum of scores for loss of acini, inflammation, necrosis, stromal fibrosis, polymorphonuclear cells (PMNs), and acinar necrosis; inflammation was measured for 10 random high power fields (HPF; 40× objective). Stromal fibrosis was evaluated in the entire pancreatic section on Masson's Trichrome-stained slides. (D) Chronic pancreatitis index scoring was quantified across all mice from each study group with healthy no CP mice having an index value of zero (not shown). N = 10 mice/group (*p < 0.05).