Figure 2
From: Cell-free DNA promotes malignant transformation in non-tumor cells
Effect of plasma cfDNA from prostate cancer patients on the expression of genes associated with the epithelial-mesenchymal transition in RWPE-1 (A) and PNT-2 (B) cells. Twenty nanograms of cfDNA were incubated with 1 × 105 cells for 24 h. Gene expression was determined by RT-qPCR and data are reported as fold change increase of gene expression compared to control cells untreated with cfDNA. The dotted line represents control expression set to 1. Results are expressed as mean ± S.D, (*p < 0. 05 and **p < 0.01; Kruskal–Wallis test). (C) Western blotting analyses of CD44 isoforms and MMP9 in RWPE-1 and PNT-2 cells. The GAPDH protein was used as an endogenous control. The full-length blots are presented in Supplementary Fig. S4.
