Figure 7
From: Cell non-autonomous requirement of p75 in the development of geniculate oral sensory neurons
Innervation of fungiform taste buds is not altered in Phox2b-Cre; p75f/f mice. The anterior tongues of Phox2b-Cre; p75fx/fx and Phox2b-WT; p75fx/fx mice were immunolabeled with antibodies to Tuj1 (green), P2X3 (blue) and K8 (red) to identify all axons, chemosensory axons and taste buds, respectively. (A–D) Tuj1, P2X3 and K8 immunolabeling of a typical fungiform taste bud from Phox2b-WT; p75fx/fx mice. (E–H) The same immunolabeling method is shown in a typical fungiform taste bud from Phox2b-Cre; p75fx/fx mice. Fungiform papillae were imaged from three independent mice per group (n = 83 taste buds in Phox2b-WT; p75fx/fx mice and n = 57 taste buds in Phox2b-Cre; p75fx/fx mice) and the amount of innervation measured. No differences were observed in either the number of P2X3 + pixels within K8 + regions (I; p = 0.64) or in the number of P2X3 + pixels within fungiform papillae (J, p = 0.42). Graphs indicate mean ± SEM. Scale bar = 25 μm.
