Figure 5
From: K63-linked ubiquitylation induces global sequestration of mitochondria
K63-linked ubiquitin chains are insufficient to induce mitophagy. (A, B) Representative Western blots of whole cell lysates of HeLa cells stably expressing mitoGFP-Sub transiently transfected with mCherry-Parkin, mCherry-ProxE3 or mCherry-ProxE3*. Cells were either left untreated or were treated for 16 h or 24 h with 10 µM CCCP or 400 nM AP21967. Quantification of the relative band intensities for TIM50 (C), TOM20 (D) and mitoGFP-Sub (E). Data were normalized to the loading control GAPDH and are expressed as fold change compared to untreated cells. Data shown represent the mean ± SEM of three (TOM20, mitoGFP-Sub) or four (TIM50) independent experiments. **p < 0.01, *p < 0.05 (one-way ANOVA, Sidak’s multiple comparison test).
