Figure 1 | Scientific Reports

Figure 1

From: Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRs

Figure 1

Characterization of replicative senescence in HUVECs. (a) Cumulative population doubling (cPD) curves. Metformin treatment was started after passage 7 (cPD = 9.83) and conducted at each medium replacement until complete growth arrest. (b) CDKN2A mRNA relative expression in young, SEN and SEN + M. Data are mean ± SD. *p < 0.05; **p < 0.01. (c) Representative positivity and quantification of the SA β-Gal staining in young, senescent (passage 16, SEN) and SEN HUVECs treated with metformin (SEN + M). HUVECs human umbilical vein endothelial cells, SA senescence-associated.

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