Figure 3
From: Long-term exposure of human endothelial cells to metformin modulates miRNAs and isomiRs
IsomiR analysis of senescent HUVECs treated with metformin. (a) Pie chart showing the proportion of isomiR variations in SEN + M samples. (b) Heatmaps showing clustering of samples, and isomiRs upregulated (left panel) and downregulated (right panel) in SEN + M compared to SEN, with a FC ≥ 1.5 and FDR < 0.05 cut-off. Data is shown following Z-score transformation. Red color indicates Z-scores > 0 (above mean), blue color indicates Z-scores < 0 (below mean). IsomiR labels are marked and colored in red or blue according to the upregulation or downregulation of their parent miRNA in SEN + M, respectively. Green lines connect variations of the same miRNA (labeled in bold) showing opposite modulation between SEN + M and SEN. IsomiRs are ordered by the MIMAT ID of the parent miRNA. For the isomiR nomenclature, the reader is referred to the Materials and Methods section. (c) Diagram reporting the frequencies of the different type of variations among isomiRs modulated by metformin treatment.
