Figure 6
From: T cell signaling and Treg dysfunction correlate to disease kinetics in IL-2Rα-KO autoimmune mice
IL-2Rα-KO T cells respond differentially to cytokine stimulation. (A) Log2 fold change of S6 phosphorylation by mean fluorescent intensity (MFI) from unstimulated over time following TCR stimulation comparing WT versus IL-2Rα-KO mice for CD4 and CD8 T cells. n = 2–4 mice per experimental group from 5 independent experiments. (B) MFI of phosphorylated STAT5 in 19-day-old WT and IL-2Rα-KO splenic T cells stimulated with rhIL-2 for 15 min with the indicated dosage. n = 2–3 mice per experimental group. (C) MFI of phosphorylated STAT5 in 19-day-old WT and IL-2Rα-KO splenic T cells stimulated with rmIL-15 for 15 min with the indicated dosage. n = 2–4 mice per experimental group from 2 independent experiments. (D) MFI of STAT5 phosphorylation in 19-day-old WT and IL-2Rα-KO T cells following 10 ng/mL rmIL-7 stimulation for 15 min. n = 4–8 mice per experimental group. Statistics: unpaired Student’s t test; *p < 0.05, **p < 0.01, ***p < 0.001. Non-significant comparisons are not shown. Statistics in (B) and (C) are compared to WT and colored by which IL-2Rα-KO group is being compared.
