Figure 5
Acidic pH augments MCF7 mammary carcinoma cell extracellular vesicle production. (a) Extracellular vesicles produced by MCF7 cells cultured for 24 h at pH 7.2 or 6.2 were analyzed by NanoSight tracking analysis and shown as vesicles per cell (mean + SEM, n = 3). (b) Vesicle size was measured, grouped into exosomes (30–110 nm) or microvesicles (> 110 nm), and shown as percent of the total number of vesicles (mean + SEM, n = 3). Double asterisks indicate p < 0.01 by t-test. (c) Cell migration was examined in fibroblasts cultured in serum free media with or without addition of conditioned media containing exosomes from MCF7 cells cultured for 24 h at pH 6.2 as indicated. Phase-contrast images are presented along with data shown as the percent of the wound that was filled by cells (mean + SEM, n = 3) with ns, double, and triple asterisks indicating p > 0.05, p < 0.01 and p < 0.001 by t-test, respectively.
