Figure 7
Acidic pH does not significantly affect MDA-MB-231 mammary carcinoma cell viability or extracellular vesicle production. (a) Viability of MDA-MB-231 or (d) T-47D cells was examined by MTT assay after 24 h growth at indicated pH. Data are shown as percent of control cells grown at pH 7.2 (mean + SEM, n = 8). (b) Extracellular vesicles produced by MDA-MB-231 cells cultured for 24 h at pH 7.2 or 6.2 were analyzed by NanoSight tracking analysis and shown as vesicles per cell (mean + SEM, n = 6). (c) Vesicle size was measured, grouped into exosomes (30–110 nm) or microvesicles (> 110 nm), and shown as percent of the total number of vesicles (mean + SEM, n = 3). ns indicates p > 0.05 as indicated by t-test.
