Figure 2 | Scientific Reports

Figure 2

From: Artificial miRNA mediated resistance in tobacco against Jatropha leaf curl Gujarat virus by targeting RNA silencing suppressors

Figure 2

Characterization of the N. benthamiana transgenics overexpressing the amiRNA against C1/C4, C2/C3 and V1/V2 ORFs, respectively. Representative pictures to show viral symptoms in (a) WT, (b) VA, (c) C1/C4, (d) C2/C3 and (e) V1/V2 amiRNA transgenics after 28 dpi. Arrow indicates yellow mosaic/ leaf distortion symptoms. (f) PCR amplification of genomic DNA (upper panel) and actin gene as a control (lower panel) in agroinfiltrated WT, VA and transgenics for detection of JLCuGV in infected samples (at 28 dpi). (g) HindIII digested RCA products of the agroinfiltrated C1/C4, C2/C3, V1/V2 transgenics, WT and VA for detection of JLCuGV in infected samples (at 28 dpi). (h) The qRT-PCR based estimation of viral load in the infiltrated plants of WT, VA and V1/V2 transgenics after 14, 21, 28 and 35 dpi. (i) Relative expression of amiRNA in transgenics. Values are represented as means ± SD (n = 3) and marked with different alphabets (from ‘a’ to ‘e’) to indicate a significant difference as determined by Tukey test at P ≤ 0.05 probability.

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