Table 2 The methylation patterns observed in amiRNA transgenics after virus infection.

Type of band	WT	VA	C1/C4 (L61)	C1/C4(67)	C2/C3 (L42)	C2/C3 (L43)	V1/V2 (L13)	V1/V2 (L15)
I	170	158	82	67	120	101	143	151
II	29	36	107	69	67	86	56	46
III	49	63	42	54	48	40	57	67
IV	67	58	84	125	80	88	59	51
TAB	315	315	315	315	315	315	315	315
TMB	145	157	233	248	195	214	172	164
FMB	116	121	126	179	128	128	116	118
MSAP (%)	46.03	49.84	73.97	78.73	61.90	67.94	54.60	52.06
FM (%)	36.83	38.41	40.00	56.83	40.63	40.63	36.83	37.46
HM (%)	9.21	11.43	33.97	21.90	21.27	27.30	17.78	14.60

VA and WT were used as controls. The highest values indicated by italic and lowest values indicated by bold.
Type I: Absence of methylation due to the presence of bands in both EcoRI/ HpaII and EcoRI/ MspI digest;
Type II: Bands present only in EcoRI/ MspI digestion but not in the EcoRI / HpaII digest;
Type III: Bands present in EcoRI/ HpaII digest but not in the EcoRI/ MspI digest;
Type IV: Absence of band in both enzyme combinations.
TAB total amplified bands, TMB total methylated bands, FMB fully methylated bands, FM Full methylation, HM hemi-methylation; the values of TAB = [I + II + III + IV], MB = [II + III + IV], FMB = [II + IV], MSAP% = [(MB/TAB) × 100], FM% = [(FMB/TAB) × 100], HM% = [III/TAB × 100].

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