Table 6 List of primers used for SYBR Green qPCR.

From: Reduced occludin and claudin-7 expression is associated with urban locations and exposure to second-hand smoke in allergic rhinitis patients

Target gene

Accession number

Forward primer (5′-3′)

Reverse primer (5′-3′)

Amplicon size (bp)

Primer spans exon junction

OCLN

NM_002538.3

CGAGGAGTGGGTTAAAAATGTGTCT

GCTTGTCATTCACTTTGCCATT

122

Yes-Forward

CLDN3

NM_001306.4

CCACGCGAGAAGAAGTACACG

AGACGTAGTCCTTGCGGTCGTA

106

No†

CLDN7

NM_001307.6

TTTTCATCGTGGCAGGTCTTG

CCCTGCCCAGCCAATAAAGA

140

Yes-Forward

TSLP

NM_033035.5

GAAACTCAGATAAATGCTACTCAGG

TCAGTAAAGGTCGATTGAAGC

127

Yes-Forward

DSG3

NM_001944.3

AGTGCCTCAAACTCACTGGT

ACGGACTTCCCCAGTGTTTC

150

Yes-Forward

GAPDH

NM_002046.7

TCGGAGTCAACGGATTTGGT

TTCCCGTTCTCAGCCTTGAC

181

Yes-Forward

  1. †CLDN3 has only one exon, thus the primer was designed within the exon.
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