Figure 2 | Scientific Reports

Figure 2

From: Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers

Figure 2

The 3D structure of the wild-type flagellin FliC, the genetically engineered FliC-NikRC and the FliC-NikRC tetramer that is spontaneously formed in solution. Each monomer unit of the FliC-NikRC tetramer has one HA Ni-binding site. Note that the D0 and partly the D1 domain forms a disordered region displaying high flexibility. While the structure of FliC (PDB ID: 1UCU) and NikR C-domain (PDB ID: 3BKU) is well established by electron microscopy, the shown structure of monomeric and tetrameric FliC-NikRC is based on modeling using the Chimera Software65. Due to the conformational variability in its disordered arms, only a hypothetical structure could be shown for the FliC-NikRC tetramer.

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