Table 1 Thermodynamic parameters for the best fit of ITC binding curve by the “Sequential Binding Sites” model.

From: Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers

 

Kd (M)

ΔH (cal/mol)

ΔS (cal/mol/deg)

HA binding site

62.5 × 10–9

 − 6301

11.8

LA binding site 1

2.13 × 10–6

 − 3897

12.9

LA binding site 2

8.93 × 10–6

 − 3731

10.6

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