Figure 1
(A) Retinal layers segmented within the HRA/Spectralis Viewing module – manual correction was performed when necessary. (B) Individual retinal layer thicknesses across each group (i.e. variables that were significantly associated with average retinal layer thickness, such as age or sex). The Spectralis 8 × 8 grids are centred on the fovea with each grid location covering 0.74 mm2 (approximately 3° × 3°). Values presented are a sample of GCL thicknesses in µm. For each group, averaged thickness were then determined at each grid location (middle). (C) Values were then clustered by statistically similar thicknesses based on applied criterion of at least 2SD separation (d′ ≥ 2) between cluster means. The resultant spatial cluster topography is generated by the various pseudo-colours. Note all images are in right eye format, demonstrated by the location of the optic nerve. Scale bar is provided in the bottom left. (D) A visual and numerical break-down of the individual clusters, demonstrating a concentric pattern. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL+HFL, outer nuclear layer + Henle’s fibre layer; IS, photoreceptor inner segment layer; OS, photoreceptor outer segment layer; RPE, retinal pigment epithelium. C1…6 = Clusters 1…6.
