Figure 3

Elevated expression of GPC3 and FAT1 in HCC cell lines. (A) The mRNA level of GPC3 in two primary liver tissues and HCC cell lines (HepG2, Hep3B and Huh7) measured by quantitative RT-PCR. (B) The mRNA level of FAT1 in the same samples. (C) GPC3 protein level of the corresponding tissues and cell lines. Full-length blots/gels were presented in Supplementary Figure S3A. (D) FAT1 protein level from the same samples. Full-length blots/gels were presented in Supplementary Figure S3B (E) Correlation of GPC3 and FAT1 protein level in three HCC cell lines (HepG2, Hep3B and Huh7). Data represent mean ± SEM (n = 3).