Figure 3
From: Modulation of cAMP metabolism for CFTR potentiation in human airway epithelial cells
Concentration–response analysis of two different cAMP-elevating agents on CFTR activity. Calu-3 cells were exposed to G protein-coupled receptor-independent cAMP inducer (a) forskolin and G protein-coupled receptor-dependent cAMP inducer (b) isoproterenol at increasing concentrations while measuring CFTR activity using an in vitro CFTR membrane potential assay. EC50 values were analyzed and reported. Each concentration–response curve was normalized to baseline over DMSO vehicle control with data presented as ± standard deviations (n = 8).
