Figure 6
From: Modulation of cAMP metabolism for CFTR potentiation in human airway epithelial cells
Consequences of PDE-4 inhibition on CFTR function using receptor-independent (forskolin) and receptor-dependent (isoproterenol) cAMP inducers. PDE-4 was inhibited with Roflumilast and Rolipram at submaximal concentrations. Forskolin-stimulated (a,b) CFTR activity in the presence of (a) Roflumilast (1 μM) and (b) Rolipram (10 μM). Isoproterenol-stimulated (c,d) CFTR activity in the presence of (c) Roflumilast and (d) Rolipram. Analysis of concentration–response curves was performed for (e) AUC, (f) EC50, (g) Emax, and (h) Baseline. Each concentration–response curve was normalized to baseline over DMSO vehicle control. All data presented as means ± standard deviations (n = 5). *P ≤ 0.05.
