Figure 7
From: Modulation of cAMP metabolism for CFTR potentiation in human airway epithelial cells
Consequences of ABCC4 and PDE-4 inhibition on CFTR function in the presence of CFTR modulator VX-770 using receptor-independent (forskolin) and receptor-dependent (isoproterenol) cAMP inducers. ABCC4 and PDE4 were inhibited with MK-571 (1.8 μM) and Roflumilast (1 μM) respectively prior to VX-770 (1 μM) stimulation. Forskolin-stimulated (a,c) CFTR activity in the presence of (a) MK-571 + VX-770 and (c) Roflumilast + VX-770. Isoproterenol-stimulated (b,d) CFTR activity in the presence of (b) MK-571 + VX-770 and (d) Roflumilast + VX-770. Analysis of concentration–response curves was performed for (e) AUC, (f) EC50, and (g) Emax. Each concentration–response curve was normalized to baseline over DMSO vehicle control. All data presented as means ± standard deviations (n = 6–7, MK-571 + VX-770 and Roflumilast + VX-770). **P ≤ 0.01; ***P ≤ 0.001.
