Table 1 Number of replicates for all experiments.
From: Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE
Cell lines | Coating | Biological n | Replicates/coating (n) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
Technical replicates (total) | ||||||||||
24 h | Wk 3 | Wk 4 | Wk 6 | Wk 7–8 | Wk 9–10 | Wk 13 | ||||
Cell adhesion force | hESC-08/017 | Col | 3 | 6–9 [m] | ||||||
LN | 3 | 6–9 [m] | ||||||||
Col + LN | 3 | 6–9 [m] | ||||||||
Col + LN + 1xNid | 3 | 6–9 [m] | ||||||||
Cell area | hESC-08/017 | Col | 1 | 80 [c] | ||||||
LN | 1 | 168 [c] | ||||||||
Col + LN | 1 | 241 [c] | ||||||||
Col + LN + 1xNid | 1 | 266 [c] | ||||||||
Morphology | hESC-08/017, hESC-11/013, hESC-13/012 | All coatings | n/a | n/a | n/a | |||||
TER | hESC-08/017, hESC-11/013, hESC-13/012 | Col | 6 | 12 [m] | 32 [m] | 34 [m] | 46 [m] | 56 [m] | ||
LN | 3 | 12 [m] | 18 [m] | 20 [m] | 12 [m] | 28 [m] | ||||
Col + LN | 6 | 24 [m] | 44 [m] | 44 [m] | 46 [m] | 52 [m] | ||||
Col + LN + 1xNid | 4 | 24 [m] | 38 [m] | 36 [m] | 48 [m] | 36 [m] | ||||
IF | hESC-08/017, hESC-11/013 | LN | 2 | 2 [s] | ||||||
Col + LN | 2 | 2 [s] | ||||||||
Col + LN + 1xNid | 2 | 2 [s] | ||||||||
Phagocytosis assay | hESC-08/017 | LN | 1 | 5 [i] 519 [c] | ||||||
Col + LN | 1 | 5 [i] 540 [c] | ||||||||
Col + LN + 1xNid | 1 | 5 [i] 528 [c] | ||||||||
Col + LN + 10xNid | 1 | 5 [i] 510 [c] | ||||||||
Ca-imaging | hESC-08/017 hESC-11/013 | LN | 2 | 465 [c] | 555 [c] | |||||
Col + LN | 2 | 473 [c] | 545 [c] | |||||||
Col + LN + 1xNid | 2 | 591 [c] | 643 [c] | |||||||
Col + LN + 10xNid | 2 | 551 [c] | 531 [c] | |||||||
Pigmentation assay | hESC-08/017 | LN | 1 | 5 [i] | ||||||
Col + LN | 1 | 5 [i] | ||||||||
Col + LN + 1xNid | 1 | 5 [i] | ||||||||
Col + LN + 10xNid | 1 | 5 [i] | ||||||||
