Figure 2
Validation of microarray results by quantitative reverse transcription PCR (qRT-PCR) (a,b) qRT-PCR validation of 25 down-regulated genes identified by microarray analysis. (a) Genes with similar expression patterns were clustered and visualized by Treeview. (b) Average fold changes in expression levels of genes identified as down-regulated genes from microarray analysis were analyzed by qRT-PCR. *p < 0.05; **p < 0.01; ***p < 0.001 by Student’s t-test, n = 5 pairs. (c,d) mRNA expression levels of genes identified as up-regulated genes from microarray analysis were not statistically different between genotypes. Treeview representation (c) and average fold changes (d) of expression determined by qRT-PCR.
