Figure 7 | Scientific Reports

Figure 7

From: PDGFRα mediated survival of myofibroblasts inhibit satellite cell proliferation during aberrant regeneration of lacerated skeletal muscle

Figure 7The alternative text for this image may have been generated using AI.

Local cues and activation of transcription factors. (A) Gene expression analysis. Quantitative gene expression analysis of the injured tissues shows relative mRNA of Il-1β and Tnfα expressions at days 7, 14 and 21 post muscle injury. (B) NF-κB activation in MFs of injured muscle. Nuclear translocation of p65 subunit at different times of the injury is shown. (C) Effect of Tyrphostin AG-370 on MFs in vitro. MFs are isolated from day 21 injured muscles and cultured for 24 h in the presence of plain medium (sham control) and Tyrphostin (20 μM). The resultant cells were analysed for the localization of p65NF-κB, Foxo3a and Caspase3 using specific antibodies. The bar diagrams show respective responses, determined on the basis of morphometric analyses of 6 fields each (20× magnification) using the ImageJ software. Data were presented as mean ± SEM. ***p < 0.001, **p < 0.01.

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