Figure 5

CR6-interacting factor 1 (CRIF1) downregulation leads to decreased cell migration measured by transwell assay in normal fibroblasts (NFs) and keloid fibroblasts (KFs). (A) NFs were transfected with CRIF1 siRNA in a dose-dependent manner and transwell assay was conducted to determine cell migration with or without MMC treatment. Scale bar 200 μm. (B) KFs were transfected with CRIF1 siRNA in a dose-dependent manner and transwell assay was conducted to determine cell migration with or without MMC treatment. Scale bar 200 μm. Quantification of migrated cells was performed using ImageJ software. All data are presented as means ± SD of three independent experiments. *P < 0.05 compared to siCON.