Figure 3 | Scientific Reports

Figure 3

From: Autophagy is deregulated in cancer-associated fibroblasts from oral cancer and is stimulated during the induction of fibroblast senescence by TGF-β1

Figure 3

Autophagy is disrupted in CAFs from GU-OSCCs. (a) The expression of both ATG5 and BECN1/ATG6 mRNAs between all three groups of fibroblasts was not statistically different (ANOVA). The expression of ATG5 and BECN1/ATG6 was normalised to ESD and the data were expressed as mean ± SD of triplicates. The data presented are representative of two independent experiments. The expression of a number of ATG genes derived by RNAseq experiment is presented in Supplementary Fig. S3. (b) Expression of LC3B-II and p62 protein in CAFs and NHOFs, as determined by Western blotting. The images shown are representative of three independent experiments. Full-length blots are presented in Supplementary Fig. S8. (c) Mean densitometric values from three experiments were normalised to GAPDH and expressed relative to NHOF1 (= 1).

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