Figure 5 | Scientific Reports

Figure 5

From: Autophagy is deregulated in cancer-associated fibroblasts from oral cancer and is stimulated during the induction of fibroblast senescence by TGF-β1

Figure 5

TGF-β1 induces autophagic flux, activation and senescence in normal fibroblasts. (a) Induction of autophagic flux, α-SMA expression and SIRT1 downregulation following treatment of NHOF1 with 4 ng/mL TGF-β1 for 5 days. Densitometric values are normalised to GAPDH. (b) Progressive increase of SA-β-gal activity in NHOF1 and NHOF2 following TGF-β1 treatment for 10 days. (c) Western blotting for autophagic (p62 and LC3B-II), activation (α-SMA) and senescent (SIRT1) markers following treatment of normal fibroblasts (NHOF1 and NHOF2) with 4 ng/mL TGF-β1 for 10 days. TGF-β1 induced autophagic flux, activation and senescence with autophagic flux preceding activation, followed by senescence in normal fibroblasts. The densitometric data are expressed as relative expression normalised to GAPDH. The Western blots are representative of three independent experiments. Full-length blots are presented in Supplementary Fig. S8.

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