Figure 5
From: Wear of hip prostheses increases serum IGFBP-1 levels in patients with aseptic loosening
IGFBP-1 production by primary cultures of human osteoblasts. (A) IGFBP-1 secretion (left panel) and IGFBP1 mRNA levels (right panel) in osteoblasts exposed (+) or not (−) to 5 mg/mL of titanium (Ti) particles for 24 h. (B–D) IGFBP-1 secretion in osteoblasts incubated for 24 h in conditioned media from osteoblasts exposed (CMTi) or not (CM) to 5 mg/mL of Ti particles (B); in conditioned media from M1 and M2 macrophages exposed (CMTi) or not (CM) to 5 mg/mL of Ti particles (C); and in conditioned media from pre-activated, LPS-treated M1 or M2 macrophages containing (CM* + Ti) or not (CM*) 5 mg/mL of Ti particles (D). Data are presented as mean + SD and expressed as relative fold induction to levels in untreated osteoblasts, which were given the arbitrary value of 1. These levels corresponded to 6.36 pg IGFBP-1 in (A), 6.18 pg of IGFBP-1 in (B), 4.99 pg in (C) and 5.45 pg in (D) per µg of total proteins. *p < 0.05 as compared with osteoblasts not exposed to particles, and #p < 0.05 as compared with Ti-treated osteoblasts not incubated in conditioned media.
