Figure 3

Error bar plots showing comparisons between the early and senescent hMSCs passages for six donors on (A) fluorescent-based \(\upbeta \)-galactosidase staining through C\({_{12}}\)FDG mean (B) forward scatter mean (C) total autofluorescence mean (D) lipopigment autofluorescence mean. Error bar plots were produced based on at least 10 cells measured per sample (experimental repeats n = 10) and 100 cells involved per autofluorescence run, and \(10^5\) cells per flow cytometry measurement. Statistical analysis: one-tailed unequal variance t-test was performed between early and senescent data. Pairs with significant differences are marked with ***(P \(\le \) 0.001), **(P \(\le \) 0.01), *(P \(\le \) 0.05) and ns indicates no significance (P > 0.05).