Figure 4 | Scientific Reports

Figure 4

From: Multi-pronged approach to human mesenchymal stromal cells senescence quantification with a focus on label-free methods

Figure 4

Fold difference (A–D) and correlation (E–H) plots of the various senescence quantification methods of hMSCs benchmarking with the \(\upbeta \)-galactosidase staining method. (A) fluorescent-based \(\upbeta \)-galactosidase staining through C\({_{12}}\)FDG mean (B) forward scatter mean (C) total autofluorescence mean (D) lipopigment autofluorescence mean. E—early, S—senescent passages. Spearman’s correlation coefficient, a statistical measure of the strength of a monotonic relationship between paired data, was employed to benchmark the various autofluorescence and flow cytometry methods with \(\upbeta \)-galactosidase.

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