Figure 3

In situ hybridization of PeMADS28 in developing ovules of P. equestris. (a,b) Placenta with ovule primordium at 4 DAP; (c) placenta with developing ovule at 32 DAP; (d) enlarged region of the dark arrow in (c); (e) placenta with developing ovule at 40 DAP; (f,g) developing ovules at 48 DAP; (h,i) developing ovule at 56 DAP. In (a), (c), (d), (e), (f) and (h), antisense probes were used to detect PeMADS28 transcripts. In (b), (g) and (i), hybridization involved sense probes (negative controls). Bars, 0.1 mm. p, placenta; op, ovule primordium; do, developing ovule; ii, inner integument; oi, outer integument; nu, nucellus; me, megaspores; DAP: days after pollination. The nucellus is highlighted by the red dash line.