Figure 4
From: Orchid Bsister gene PeMADS28 displays conserved function in ovule integument development
Localization patterns of PeMADS28-GFP fusions in Phalaenopsis protoplasts. Images show fluorescence and bright-field confocal microscopy images and merged images of flower protoplast. (a) Empty vector was no green fluorescence in the cytoplasm and nucleus. (b) Cell in (a) stained with propidium iodide (PI) represented in red to confirm the nucleus. (c) Cell in (a) and (b) by bright-field confocal microscopy. (d) Merged image of (a), (b) and (c) to confirm green fluorescence in the cytoplasm of a flower cell. (e) PeMADS28-GFP green fluorescence in nucleus and cytoplasm. (f) Cell in (e) stained with PI represented in red to confirm the nucleus. (g) Cell in (e) and (f) by bright-field confocal microscopy. (h) Merged image of (e), (f) and (g) to confirm green fluorescence in cytoplasm. Bars: 20 µm.
