Figure 6

OD600, CDW, urease activity and specific urease activity of shake flask cultivations of S. pasteurii in different media, corresponding to the OTR and backscatter data in Fig. 5. CDW was calculated with a predetermined correlation from OD600 data. The chemically defined medium is described in Table 1. The improved chemically defined medium is supplemented with glutamate group amino acids. The complex medium corresponds to the YE medium. OTR and backscatter data from cultivation with the simple YE medium can be found in Supplementary Figure S11. The improved complex medium is supplemented YE medium with glucose, phosphate, trace elements, thiamine-HCl, nicotinic acid, L-methionine, L-cysteine and glutamate group amino acids, according to the concentrations of Table 1, based on the results of Fig. 4b (purple line). Here, arithmetic mean values derived from technical replicates (N = 3) are shown. The error bars depict the standard deviation.