Table 1 Composition of the chemically defined medium.
Ingredients | Concentration | Final |
|---|---|---|
Stock solution (g/L) | Concentration (g/L) | |
Main components | ||
Glucose monohydrate | 11.00 | |
Sodium acetate trihydrate | 1.660 | |
K\(_{2}\)HPO\(_{4}\) | 1.700 | |
Urea | 20.00 | |
NaCl | 5.000 | |
Ammonia stock solution: 20 mL/L | ||
\((\hbox {NH}_4)_2\hbox {SO}_4\) | 374.50 | 7.490 |
Trace elements | ||
Iron stock solution: 5 mL/L | ||
\(\hbox {FeCl}_3\cdot 6\hbox {H}_2\hbox {O}\) | 1 | 0.005 |
\(\hbox {FeCl}_{2}\) | 1 | 0.005 |
Micronutrient stock solution: 50 mL/L | ||
\(\hbox {MgCl}_2\cdot 6\hbox {H}_2\hbox {O}\) | 8.54 | 0.427 |
\(\hbox {MnSO}_4\cdot 4\hbox {H}_2\hbox {O}\) | 0.56 | 0.028 |
\(\hbox {ZnSO}_4\cdot 7\hbox {H}_2\hbox {O}\) | 0.18 | 0.009 |
\(\hbox {CoSO}_4\cdot 7\hbox {H}_2\hbox {O}\) | 0.085 | 0.004 |
\(\hbox {CuSO}_4\cdot 5\hbox {H}_2\hbox {O}\) | 0.08 | 0.004 |
\((\hbox {NH}_4)_6\hbox {Mo}_7\hbox {O}_{24}\cdot 4\hbox {H}_2\hbox {O}\) | 0.06 | 0.003 |
\(\hbox {NiCl}_2\cdot 6\hbox {H}_2\)O | 0.2 | 0.010 |
EDTA | 0.2 | 0.010 |
Glutamate group | ||
L-glutamine (Gln) | 1.000 * | |
Glutamate stock solution: 125 mL/L | ||
L-histidine (His) | 1.2 | 0.150 * |
L-proline (Pro) | 5.4 | 0.675 * |
L-arginine (Arg) | 1 | 0.125 * |
L-glutamic acid stock solution: 250 mL/L | ||
L-glutamic acid (Glu) | 2 | 0.500 * |
Aspartate group | ||
Aspartate stock solution: 250 mL/L | ||
L-aspartic acid (Asp) | 1.68 | 0.420 |
L-isoleucin (Ile) | 0.84 | 0.210 |
L-threonin (Thr) | 0.9 | 0.225 |
L-Methionine stock solution: 20 mL/L | ||
L-methionine (Met) | 6.25 | 0.125 |
Serine group | ||
Serine stock solution: 20 mL/L | ||
L-cysteine (Cys) | 6.5 | 0.130 |
Glycine (Gly) | 8.75 | 0.175 |
L-serine stock solution: 20 mL/L | ||
L-serine (Ser) | 17 | 0.340 |
Aromatic group | ||
L-tyrosine (Tyr) | 0.250 | |
Aromatic stock solution: 20 mL/L | ||
L-phenylalanine (Phe) | 13.75 | 0.275 |
L-tryptophan (Trp) | 2.5 | 0.050 |
Pyruvate group | ||
Pyruvate stock solution: 40 mL/L | ||
L-alanine (Ala) | 6 | 0.240 |
L-leucine (Leu) | 11.875 | 0.475 |
L-lysine monohydrate (Lys) | 12.356 | 0.494 |
L-valine (Val) | 8.125 | 0.325 |
Vitamins | ||
Vitamin stock solution: 100 mL/L | ||
Biotin (B7) | 0.03 | 0.003 |
Thiamin\(\cdot\)HCl (B1) | 0.01 | 0.001 |
Nicotinic acid (B3) | 1 | 0.100 |
Folic acid (B9) | 0.01 | 0.001 |
Calciumpantothenate (B5) | 0.03 | 0.003 |
